YYT42–Isivavanyeli sokuNgena kweeAerosols ezingcoliswe yiBiological
Jonga la manani alandelayo ngelixa ufunda esi sahluko.
Imigangatho
I-ISO/DIS 22611 Iimpahla zokuzikhusela kwiintsholongwane-Indlela yokuvavanya ukumelana nokungena kwee-aerosols ezingcoliswe yibhayoloji.
Iinkcukacha
Umatshini wokuvelisa i-aerosol: I-Atomizer
Igumbi lokubonakalisa:I-PMMA
Indibano yesampulu:2, intsimbi engagqwaliyo
Impompo yeVacum:Ukuya kuthi ga kwi-80kpa
Ubukhulu: 300mm*300mm*300mm
Ukunikezwa Amandla:220V 50-60Hz
Ubungakanani bomatshini: 46cm×93cm×49cm（H）
Ubunzima obucocekileyo: 35kg
Ukulungiselela
Beka ezi ndawo zintathu kwikhabhathi yokhuseleko lwebhayoloji. Jonga indawo nganye yomatshini wokuvavanya kwaye uqinisekise ukuba zonke iindawo zisebenza kakuhle kwaye ziqhagamshelana kakuhle.
Ukusika iisampulu ezisibhozo zibe zizangqa ezinobubanzi obuyi-25mm.
Lungisa inkcubeko ye-Staphylococcus aureus ngokuhambisa intsholongwane kwi-agar yesondlo (egcinwe kwi-4±1℃) iye kwi-broth yesondlo kwaye ifakelwe kwi-37±1℃ kwi-orbital shaker.
Nciphisa inkcubeko ibe ngumthamo ofanelekileyo we-isotonic saline ecocekileyo ukuze ufumane inani lokugqibela leebhaktheriya elimalunga ne-5 * 107iiseli cm-3kusetyenziswa igumbi lokubala iintsholongwane likaThoma.
Gcwalisa inkcubeko engasentla kwi-atomizer. Inqanaba lolwelo liphakathi kwenqanaba eliphezulu nelisezantsi.
Umsebenzi
Faka indibano yesampulu. Beka i-silicone washer A, ilaphu lokuvavanya, i-silicone washer B, i-membrane, i-wire support kwisiciko esivulekileyo, ugqume ngesiseko.
Faka enye indibano yesampulu ngaphandle kwesampulu.
Vula isiciko esingaphezulu segumbi lovavanyo.
Faka indibano yesampulu kunye nesampulu kunye nendibano ngaphandle kwesampulu ngeFasten yoMfanekiso 4-1.
Qinisekisa ukuba zonke iityhubhu zidibene kakuhle.
Qhagamshela umoya ocinezelweyo ukuze ulungise umoya ocinezelweyo.
Faka umoya xa uhamba nge-5L/min ngokulungelelanisa i-flow meter kwi-atomizer uze uqale ukuvelisa i-aerosol.
Emva kwemizuzu emi-3 vula ipompo ye-vacumm. Yibeke njenge-70kpa.
Emva kwemizuzu emi-3, cima umoya kwi-atomizer, kodwa shiya ipompo ye-vacuum isebenza umzuzu o-1.
Cima ipompo ye-vaccum.
Susa iindibano zesampulu kwigumbi. Uze udlulisele iimembrane ze-0.45um ngendlela engenatyhefu kwiibhotile ze-universal ezine-10ml ye-isotonic saline ecocekileyo.
Khipha ngokushukumisa umzuzu o-1. Kwaye wenze umxube olandelelanayo nge-saline ecocekileyo. (10-1, 10-2, 10-3, kunye ne-10-4)
Beka ii-aliquots ze-1ml ze-dilution nganye zibe ziphindwe kabini usebenzisa i-nutrient agar.
Faka iipleyiti ubusuku bonke kwi-37±1℃ uze uveze iziphumo usebenzisa umlinganiselo wenani leebhaktheriya ezingasemva kwinani leebhaktheriya ezidlulisiweyo kwisampulu yovavanyo.
Yenza izigqibo ezine ngohlobo ngalunye lwelaphu okanye imeko yelaphu.
Njengakwizixhobo zombane zonke, le yunithi kufuneka isetyenziswe ngokuchanekileyo kwaye ulondolozo kunye nokuhlolwa kufuneka kwenziwe rhoqo. Amanyathelo okhuseleko anjalo aya kuqinisekisa ukusebenza ngokukhuselekileyo nangokufanelekileyo kwezixhobo.
Ukugcinwa rhoqo kuqulathe uhlolo olwenziwa ngqo ngumqhubi wovavanyo kunye/okanye ngabasebenzi benkonzo abagunyazisiweyo.
Ukugcinwa kwezixhobo luxanduva lomthengi kwaye kufuneka kwenziwe njengoko kuchaziwe kwesi sahluko.
Ukungaphumeleli ukwenza amanyathelo okulungisa acetyiswayo okanye ukugcinwa okwenziwe ngabantu abangagunyaziswanga kunokwenza iwaranti ingaphumeleli.
1. Umatshini kufuneka uhlolwe ukuze kuthintelwe ukuvuza konxibelelwano ngaphambi kovavanyo;
2. Akuvumelekanga ukuhambisa umatshini xa uwusebenzisa;
3. Khetha umbane ofanelekileyo kunye ne-voltage. Musa ukuyibeka phezulu kakhulu ukuze ungatshisi isixhobo;
4. Nceda unxibelelane nathi ukuze ukwazi ukusingatha ngexesha xa umatshini ungasebenzi kakuhle;
5. Kufuneka ibe nendawo efanelekileyo yokungenisa umoya xa umatshini usebenza;
6. Ukucoca umatshini emva kovavanyo ngalo lonke ixesha;
| Isenzo | I-WHO | Nini |
| Jonga ukuqinisekisa ukuba akukho monakalo wangaphandle kumatshini, onokuthi ubeke emngciphekweni ukhuseleko lokusetyenziswa kwawo. | Umqhubi | Ngaphambi kweseshoni nganye yokusebenza |
| Ukucoca umatshini | Umqhubi | Ekupheleni kovavanyo ngalunye |
| Ukujonga ukuvuza koqhagamshelo | Umqhubi | Ngaphambi kovavanyo |
| Ukujonga imeko kunye nokusebenza kwamaqhosha, umyalelo womqhubi. | Umqhubi | Veki nganye |
| Ukujonga ukuba intambo yombane iqhotyoshelwe kakuhle okanye akunjalo. | Umqhubi | Ngaphambi kovavanyo |
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